Journal: bioRxiv

Article Title: B7-H3-targeted natural killer cells effectively kill atypical teratoid / rhabdoid tumors and extend survival in orthotopic xenografts

doi: 10.64898/2026.01.15.699746

Figure Lengend Snippet: (A) Immunohistochemistry (IHC) staining for B7-H3 on FFPE primary tumor tissue, and (B) semi-quantitative scoring of B7-H3 expression. 20X magnification, n=8. (C) AT/RT cell line B7-H3 expression. Isotype-stained samples are shown in the histograms directly below each B7-H3-stained sample. (D) B7-H3 surface molecules per cell quantified by molecules of equivalent soluble fluorochrome (MESF) flow cytometry assay. Each dot representative of technical replicates. (E) Total B7-H3 protein expression with or without deglycosylation assessed by western blot.

Article Snippet: CAR expression analysis was performed by primary staining of NK cells with His-tagged recombinant 4Ig B7-H3 protein (Sino Biological 11188-H08H) followed by secondary staining with anti-His-PE or anti-His-APC (BioLegend).

Techniques: Immunohistochemistry, Expressing, Staining, Flow Cytometry, Western Blot

Journal: bioRxiv

Article Title: B7-H3-targeted natural killer cells effectively kill atypical teratoid / rhabdoid tumors and extend survival in orthotopic xenografts

doi: 10.64898/2026.01.15.699746

Figure Lengend Snippet: (A) CARs consisting of Hu8H9 scFv and various affinity mutants, 2B4 (CD244) hinge (H), transmembrane (TM), and intracellular domains (IC), and CD3ζ signaling domain. (B) Representative primary NK-cell CAR expression. (C) CAR expression (n=4 healthy donors, 4-9 separate experiments). (D) Fold expansion of CAR-NK cells starting at day of transduction (n=4 healthy donors). (E) Representative CAR transduced NK-cell B7-H3 expression 2-4 days post-transduction. (F) B7-H3 expression on CAR-NK cells. (n = 3 healthy donors). *** = p<0.001 UTD vs. all others. (G) Representative measurement of CAR-NK cell degranulation post 2-hour co-culture with CHLA-06 AT/RT cells detected by CD107a staining.

Article Snippet: CAR expression analysis was performed by primary staining of NK cells with His-tagged recombinant 4Ig B7-H3 protein (Sino Biological 11188-H08H) followed by secondary staining with anti-His-PE or anti-His-APC (BioLegend).

Techniques: Expressing, Transduction, Co-Culture Assay, Staining

Journal: bioRxiv

Article Title: B7-H3-targeted natural killer cells effectively kill atypical teratoid / rhabdoid tumors and extend survival in orthotopic xenografts

doi: 10.64898/2026.01.15.699746

Figure Lengend Snippet: (A) CHLA-06.ffLuc (25k cells) were injected into caudate putamen of NSG mice at day 0. Randomization/cohorting was performed at day 2, and intratumoral NK cell treatment (3 million cells per treatment) was started on day 3 and repeated on days 10 and 17. (B) Tumor radiance measured by IVIS Spectrum bioluminescent imaging and analyzed using Living Image software. (C) tumor radiance over time of CHLA-06.ffLuc bearing mice (n=8-9 per condition). (D) Kaplan-Meier survival curve. ***= p<0.001. (E) Brain tissue harvested at endpoint (D23 post-tumor, D6 post-third NK-cell treatment for both samples), representative slides from UTD NK treated and CAR-NK treated mice stained for human B7-H3 and CD45. 40X magnification.

Article Snippet: CAR expression analysis was performed by primary staining of NK cells with His-tagged recombinant 4Ig B7-H3 protein (Sino Biological 11188-H08H) followed by secondary staining with anti-His-PE or anti-His-APC (BioLegend).

Techniques: Injection, Imaging, Software, Staining

Journal: bioRxiv

Article Title: B7-H3-targeted natural killer cells effectively kill atypical teratoid / rhabdoid tumors and extend survival in orthotopic xenografts

doi: 10.64898/2026.01.15.699746

Figure Lengend Snippet: (A) BT12.ffLuc (100k cells) were injected into the right lateral ventricle of NSG mice at day 0. Randomization/cohorting was performed at day 2, and ICV NK cell treatment was started on day 3 and repeated on days 10 and 17. (B) Kaplan-Meier survival curve, and (C) percent weight change over time of ICV BT12.ffLuc bearing mice. Each dotted line represents an individual animal. Representative histology of endpoint brain and spinal tissue from (D) UTD NK treated, and (E) CAR-NK treated mice with corresponding human B7-H3 and CD45 IHC stains. Animal endpoints = 31, 62 days post-tumor injection, respectively. 200X magnification. (F) Quantification of tumor associated human NK cells. n = 5-7 per condition.

Article Snippet: CAR expression analysis was performed by primary staining of NK cells with His-tagged recombinant 4Ig B7-H3 protein (Sino Biological 11188-H08H) followed by secondary staining with anti-His-PE or anti-His-APC (BioLegend).

Techniques: Injection